Hotspot

petA-psbJ

LSC · 66207-67066 · 860 bp

Back to Mangifera

Pi 0.0103

MarkerSeek score 53.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 860
variable_sites: 5
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.56
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	TATTCCGGATTGGGTTCGTC	50.0	50.0	58.0	57.4	631-1006 bp	1.0	79.3
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	TATTCCGGATTGGGTTCGTC	50.0	50.0	58.2	57.4	628-1003 bp	1.0	78.2
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	GGATTGGGTTCGTCCTTGTA	50.0	50.0	58.0	57.2	625-1000 bp	1.0	78.0
petA-psbJ_p4	GCATCTGTTGTTTTGGCACA	TATTCCGGATTGGGTTCGTC	45.0	50.0	57.8	57.4	596-971 bp	1.0	77.9
petA-psbJ_p5	TTGGCATCTGTTGTTTTGGC	TATTCCGGATTGGGTTCGTC	45.0	50.0	57.8	57.4	599-974 bp	1.0	77.7