Hotspot

ycf4-cemA

LSC · 63668-64315 · 648 bp

Back to Mangifera

Pi 0.0201

MarkerSeek score 57.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 648
variable_sites: 13
pi: 0.0201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTTCTTGCGCGTACCAATTG	AATGGGTGCGTTCTTTTT	45.0	38.9	57.7	52.9	703 bp	1.0	41.5
ycf4-cemA_p2	TGACTCCGCGAGAAATTGAG	AATGGGTGCGTTCTTTTT	50.0	38.9	58.0	52.9	746 bp	1.0	41.4
ycf4-cemA_p3	TGAGAATTTGACTCCGCGAG	AATGGGTGCGTTCTTTTT	50.0	38.9	58.0	52.9	754 bp	1.0	41.4
ycf4-cemA_p4	GAGAATTTGACTCCGCGAGA	AATGGGTGCGTTCTTTTT	50.0	38.9	57.7	52.9	753 bp	1.0	41.4
ycf4-cemA_p5	CCGCGAGAAATTGAGCAAAA	AATGGGTGCGTTCTTTTT	45.0	38.9	57.7	52.9	741 bp	1.0	41.4