Hotspot

accD-psaI

LSC · 61825-62533 · 709 bp

Back to Mangifera

Pi 0.00705

MarkerSeek score 62.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 5
pi: 0.00705
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAGTTATTTCAGCTCCACGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	56.9	58.0	848 bp	1.0	71.5
accD-psaI_p2	GAGTTATTTCAGCTCCACGC	AAGAAGCCATTGCAATTGCC	50.0	45.0	56.9	57.9	854 bp	1.0	70.9
accD-psaI_p3	GAGTTATTTCAGCTCCACGC	ACGGCACAAAAATAGAGGGT	50.0	45.0	56.9	57.4	815 bp	1.0	67.1
accD-psaI_p4	TGAGTTATTTCAGCTCCACGC	CCATTGCAATTGCCGGAAAT	47.6	45.0	58.7	58.0	849 bp	1.0	67.0
accD-psaI_p5	TGAGTTATTTCAGCTCCACGC	AAGAAGCCATTGCAATTGCC	47.6	45.0	58.7	57.9	855 bp	1.0	66.4