Hotspot

trnS-UGA-psbC

LSC · 45845-46088 · 244 bp

Back to Mangifera

Pi 0.0205

MarkerSeek score 64.30

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 244
variable_sites: 5
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GGTTGATAGCTCCGGTCTTG	TAGGTCATTTATGGCACGCG	55.0	50.0	58.1	58.4	428 bp	1.0	77.1
trnS-UGA-psbC_p2	GGTTGATAGCTCCGGTCTTG	GCTGCAGCAGGATTTGAAAA	55.0	45.0	58.1	57.6	393 bp	1.0	77.0
trnS-UGA-psbC_p3	GATGGCCGAGTGGTTGATAG	TAGGTCATTTATGGCACGCG	55.0	50.0	58.1	58.4	439 bp	1.0	76.5
trnS-UGA-psbC_p4	GATGGCCGAGTGGTTGATAG	GCTGCAGCAGGATTTGAAAA	55.0	45.0	58.1	57.6	404 bp	1.0	76.4
trnS-UGA-psbC_p5	GGGTTCGAATCCCTCTCTCT	TAGGTCATTTATGGCACGCG	55.0	50.0	58.2	58.4	374 bp	1.0	76.0