Hotspot

trnE-UUC-trnT-UGU

LSC · 33170-34364 · 1195 bp

Back to Mangifera

Pi 0.00814

MarkerSeek score 49.70

Primer available yes

Label trnE-UUC-trnT-UGU

Feature Metadata

label_name: trnE-UUC-trnT-UGU
feature_type: igs
region: LSC
length_bp: 1195
variable_sites: 7
pi: 0.00814
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-UGU_p1	GCTGCCTCCTTGAAAGAGAG	GCGATGCTCTAACCTCTGAG	55.0	55.0	58.3	57.9	939-1273 bp	1.0	81.3
trnE-UUC-trnT-UGU_p2	TGTCCTGAACCACTAGACGA	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.7	57.9	918-1252 bp	1.0	81.2
trnE-UUC-trnT-UGU_p3	AACTTCCTTTCGTGGTACCC	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.4	57.9	984-1318 bp	1.0	79.0
trnE-UUC-trnT-UGU_p4	CCTGAACCACTAGACGATGG	GCGATGCTCTAACCTCTGAG	55.0	55.0	57.4	57.9	915-1249 bp	1.0	78.8
trnE-UUC-trnT-UGU_p5	CTGAACCACTAGACGATGGG	GCGATGCTCTAACCTCTGAG	55.0	55.0	57.4	57.9	914-1248 bp	1.0	78.8