Hotspot

trnS-GCU-trnG-UCC

LSC · 9828-10572 · 745 bp

Back to Mangifera

Pi 0.00672

MarkerSeek score 62.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 5
pi: 0.00672
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	TGAATGAAACTGAAGGACCCC	50.0	47.6	58.0	57.8	855-883 bp	1.0	71.3
trnS-GCU-trnG-UCC_p2	CCGACGCTTTAATCCACTCA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	810-838 bp	1.0	70.6
trnS-GCU-trnG-UCC_p3	CCGACGCTTTAATCCACTCA	ACGAATCGCACTTTTACCAC	50.0	45.0	58.0	56.8	810-838 bp	1.0	70.6
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	TGAATGAAACTGAAGGACCCC	50.0	47.6	58.2	57.8	913-941 bp	1.0	70.1
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.2	58.2	868-896 bp	1.0	69.4