Hotspot

rpl32-trnL-UAG

SSC · 113881-114693 · 813 bp

Back to Mandragora

Pi 0.0249

MarkerSeek score 61.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 813
variable_sites: 30
pi: 0.0249
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAATCGAACGGGGATTCCAG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.4	57.9	1540-1615 bp	1.0	79.1
rpl32-trnL-UAG_p2	GAATCGAACGGGGATTCCAG	GTCCCGAAGAAATCCAACGA	55.0	50.0	58.4	57.9	1877-1952 bp	1.0	79.0
rpl32-trnL-UAG_p3	GAATCGAACGGGGATTCCAG	CCCGAAGAAATCCAACGAGT	55.0	50.0	58.4	57.9	1875-1950 bp	1.0	78.9
rpl32-trnL-UAG_p4	GAATCGAACGGGGATTCCAG	GAAGTAGCAAAGCCTTGGGT	55.0	50.0	58.4	58.1	2028-2103 bp	1.0	78.8
rpl32-trnL-UAG_p5	GAATCGAACGGGGATTCCAG	TGAAGTAGCAAAGCCTTGGG	55.0	50.0	58.4	58.1	2029-2104 bp	1.0	78.8