Hotspot

ndhF-rpl32

SSC · 112939-113712 · 774 bp

Back to Mandragora

Pi 0.02
MarkerSeek score 67.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
774
variable_sites
23
pi
0.02
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CGGCGAAAACTTTTTGTTGC TGCTACCCAATATCCCTTCCT 45.0 47.6 57.7 57.9 971-972 bp 1.0 71.2
ndhF-rpl32_p2 GCGAAAACTTTTTGTTGCCG TGCTACCCAATATCCCTTCCT 45.0 47.6 57.7 57.9 969-970 bp 1.0 71.2
ndhF-rpl32_p3 GCGAAAACTTTTTGTTGCCG TGATGTCGAAGTGCGTTTTT 45.0 40.0 57.7 56.6 918-919 bp 1.0 68.7
ndhF-rpl32_p4 GCGAAAACTTTTTGTTGCCG TTGATGTCGAAGTGCGTTTT 45.0 40.0 57.7 56.6 919-920 bp 1.0 68.7
ndhF-rpl32_p5 GCGAAAACTTTTTGTTGCCG TTTGATGTCGAAGTGCGTTT 45.0 40.0 57.7 56.6 920-921 bp 1.0 68.7