Hotspot

rpl20-rps12

LSC · 69871-70672 · 802 bp

Back to Mandragora

Pi 0.00665

MarkerSeek score 56.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 8
pi: 0.00665
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	958-959 bp	1.0	75.4
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	957-958 bp	1.0	75.4
rpl20-rps12_p3	TTTTTGTTCGACGTCTCCGA	AAATGTCACGAAATCCCCCG	45.0	50.0	57.8	58.5	918-919 bp	1.0	74.7
rpl20-rps12_p4	TTCGACGTCTCCGAGCTATA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.8	58.5	912-913 bp	1.0	74.5
rpl20-rps12_p5	TTTTGTTCGACGTCTCCGAG	AAATGTCACGAAATCCCCCG	50.0	50.0	58.2	58.5	917-918 bp	1.0	74.5