Hotspot

accD-psaI

LSC · 59357-60121 · 765 bp

Back to Mandragora

Pi 0.0107

MarkerSeek score 58.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 12
pi: 0.0107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGTTATTTAAGCTCCACGCCT	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.4	57.9	871-885 bp	1.0	68.5
accD-psaI_p2	AGTTATTTAAGCTCCACGCCT	TGCAATTGCCGGAAAGACTA	42.9	45.0	57.4	57.8	861-875 bp	1.0	67.8
accD-psaI_p3	AGTTATTTAAGCTCCACGCCT	CCGGAAAGACTAGGCCTACT	42.9	55.0	57.4	58.2	853-867 bp	1.0	67.6
accD-psaI_p4	AGTTATTTAAGCTCCACGCCT	AGACTAGGCCTACTAACGGC	42.9	55.0	57.4	58.3	847-861 bp	1.0	66.9
accD-psaI_p5	TTTAAGCTCCACGCCTTTTT	AAGAAGCCATTGCAATTGCC	40.0	45.0	56.2	57.9	866-880 bp	1.0	66.8