Pi
0.006
MarkerSeek score
54.60
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 723-752 bp | 1.0 | 83.4 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 731-760 bp | 1.0 | 82.8 |
| trnT-UGU-trnL-UAA_p3 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 755-784 bp | 1.0 | 82.3 |
| trnT-UGU-trnL-UAA_p4 | AAGAATAGGGAAAAGCCGGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.2 | 58.0 | 779-808 bp | 1.0 | 82.2 |
| trnT-UGU-trnL-UAA_p5 | AAGAATAGGGAAAAGCCGGC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.2 | 58.1 | 787-816 bp | 1.0 | 81.7 |