Hotspot

psbM-trnD-GUC

LSC · 29662-30709 · 1048 bp

Back to Mandragora

Pi 0.0103

MarkerSeek score 61.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1048
variable_sites: 16
pi: 0.0103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGTAGAAAAGCGGTAGGAACT	CCCGTATTTCCCTGGGATTG	42.9	55.0	56.4	58.0	1203-1224 bp	1.0	60.7
psbM-trnD-GUC_p2	AGTAGAAAAGCGGTAGGAACT	TCCCGTATTTCCCTGGGATT	42.9	50.0	56.4	58.1	1204-1225 bp	1.0	60.1
psbM-trnD-GUC_p3	AAGTAGAAAAGCGGTAGGAACT	CCCGTATTTCCCTGGGATTG	40.9	55.0	57.1	58.0	1204-1225 bp	1.0	58.5
psbM-trnD-GUC_p4	AGAAAAGCGGTAGGAACTAGA	CCCGTATTTCCCTGGGATTG	42.9	55.0	56.1	58.0	1200-1221 bp	1.0	58.5
psbM-trnD-GUC_p5	AAGTAGAAAAGCGGTAGGAACT	TCCCGTATTTCCCTGGGATT	40.9	50.0	57.1	58.1	1205-1226 bp	1.0	57.9