Hotspot

trnK-UUU-rps16

LSC · 4029-4549 · 521 bp

Back to Mandragora

Pi 0.0154
MarkerSeek score 66.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
521
variable_sites
12
pi
0.0154
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAGCCGAGTACTCTACCGTT TTGAAAAAGGGGCTCAACCT 50.0 45.0 58.2 57.5 652-661 bp 1.0 76.4
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TTGAAAAAGGGGCTCAACCT 50.0 45.0 58.2 57.5 653-662 bp 1.0 76.3
trnK-UUU-rps16_p3 TCAGTCGTGGTCTTCCAAAC TTGAAAAAGGGGCTCAACCT 50.0 45.0 57.8 57.5 738-747 bp 1.0 75.7
trnK-UUU-rps16_p4 CAGTCGTGGTCTTCCAAACT TTGAAAAAGGGGCTCAACCT 50.0 45.0 57.8 57.5 737-746 bp 1.0 75.6
trnK-UUU-rps16_p5 AAAAGACACTAGCCGCACTT TTGAAAAAGGGGCTCAACCT 45.0 45.0 57.7 57.5 674-683 bp 1.0 75.6