Hotspot

rps18

LSC · 54-431 · 378 bp

Back to Mammillaria

Pi 0.0132

MarkerSeek score 61.70

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 378
variable_sites: 5
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	CCAGTTGAAGAACCCAAGCA	TGAAAACAAACCAGCAATCCA	50.0	38.1	58.3	56.8	487 bp	1.0	60.9
rps18_p2	CCCCAGTTGAAGAACCCAAG	TGAAAACAAACCAGCAATCCA	55.0	38.1	58.4	56.8	489 bp	1.0	60.3
rps18_p3	TGAAGAACCCAAGCAAGAGC	TGAAAACAAACCAGCAATCCA	50.0	38.1	58.4	56.8	482 bp	1.0	60.3
rps18_p4	CCAGTTGAAGAACCCAAGCA	TTGAAAACAAACCAGCAATCCA	50.0	36.4	58.3	57.5	488 bp	1.0	58.5
rps18_p5	CCCCAGTTGAAGAACCCAAG	TTGAAAACAAACCAGCAATCCA	55.0	36.4	58.4	57.5	490 bp	1.0	58.0