Hotspot

psaC

SSC · 35212-35457 · 246 bp

Back to Mammillaria

Pi 0

MarkerSeek score 25.10

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TCGTCTGGTATTTGAATATGTGGT	GGTACGGGTTTTTGTCGGTA	37.5	50.0	58.0	57.8	411-420 bp	1.0	48.5
psaC_p2	CTGGTAAATCATTGATTCGTCTGG	GGTACGGGTTTTTGTCGGTA	41.7	50.0	57.9	57.8	427-436 bp	1.0	48.1
psaC_p3	TGGTAAATCATTGATTCGTCTGG	GGTACGGGTTTTTGTCGGTA	39.1	50.0	56.9	57.8	426-435 bp	1.0	48.0
psaC_p4	GGTAAATCATTGATTCGTCTGGT	GGTACGGGTTTTTGTCGGTA	39.1	50.0	56.9	57.8	425-434 bp	1.0	48.0
psaC_p5	CGTCTGGTATTTGAATATGTGGT	GGTACGGGTTTTTGTCGGTA	39.1	50.0	56.7	57.8	410-419 bp	1.0	46.1