Hotspot

atpH-atpI

LSC · 65135-65624 · 490 bp

Back to Mammillaria

Pi 0.00816

MarkerSeek score 61.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 4
pi: 0.00816
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.0	57.9	577 bp	1.0	79.2
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TACCTGTCATGTTCCTCGGA	45.0	50.0	58.0	57.8	626 bp	1.0	78.4
atpH-atpI_p3	CAGGCCCAATAGAAGCAAGT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.9	57.9	618 bp	1.0	78.4
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.9	57.9	593 bp	1.0	78.3
atpH-atpI_p5	GCAGCAGTACCTTGACCAAT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	58.2	57.9	640 bp	1.0	78.0