Hotspot

ndhB-ycf2

SSC · 50933-51210 · 278 bp

Back to Mammillaria

Pi 0.054

MarkerSeek score 67.60

Primer available yes

Label ndhB-ycf2

Feature Metadata

label_name: ndhB-ycf2
feature_type: igs
region: SSC
length_bp: 278
variable_sites: 15
pi: 0.054
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-ycf2_p1	GGTTCGCTCGAGAAATTCCT	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	878-985 bp	1.0	82.8
ndhB-ycf2_p2	CGGGAATTTCAATTGCGCTT	GTTGGGCGCTTTAACCATTC	45.0	50.0	58.0	58.0	980-1087 bp	1.0	82.6
ndhB-ycf2_p3	GGTTCGCTCGAGAAATTCCT	CGATCAGACCAACCTTCTGG	50.0	55.0	58.0	58.0	706-829 bp	1.0	81.7
ndhB-ycf2_p4	CGGGAATTTCAATTGCGCTT	CGATCAGACCAACCTTCTGG	45.0	55.0	58.0	58.0	808-931 bp	1.0	81.5
ndhB-ycf2_p5	CCTGTTGTTCCGATGGAGAG	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.0	58.0	1443 bp	0.5	59.8