Hotspot

psaC-ndhD

SSC · 35458-35609 · 152 bp

Back to Mammillaria

Pi 0.106

MarkerSeek score 91.70

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 152
variable_sites: 15
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 91.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	TTTAGGGCCTGAAACAACCC	AGAACCTGCAAATATGGGCA	50.0	45.0	58.0	57.5	242-252 bp	1.0	83.8
psaC-ndhD_p2	CCGGAGTATGGGTCTAGCTT	AGAACCTGCAAATATGGGCA	55.0	45.0	58.3	57.5	224-234 bp	1.0	82.1
psaC-ndhD_p3	TGTGAATCTGCCTGTCCAAC	AGAACCTGCAAATATGGGCA	50.0	45.0	58.1	57.5	286-296 bp	1.0	81.8
psaC-ndhD_p4	TGCCTGTCCAACCGATTTTT	AGAACCTGCAAATATGGGCA	45.0	45.0	58.3	57.5	278-288 bp	1.0	80.4
psaC-ndhD_p5	CAGAGGACTGTGTTGGTTGT	AGAACCTGCAAATATGGGCA	50.0	45.0	57.7	57.5	312-322 bp	1.0	79.4