Hotspot

rpoA-rps11

LSC · 9324-9394 · 71 bp

Back to Mammillaria

Pi 0.0423

MarkerSeek score 76.30

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 3
pi: 0.0423
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	TTCAACACACTTCCACTGCA	CGGAAGGGATGGAGCATTAC	45.0	55.0	57.9	58.1	246 bp	1.0	79.1
rpoA-rps11_p2	TTCAACACACTTCCACTGCA	GAAGGGATGGAGCATTACGG	45.0	55.0	57.9	58.1	244 bp	1.0	79.1
rpoA-rps11_p3	TTCAACACACTTCCACTGCA	GGAAGGGATGGAGCATTACG	45.0	55.0	57.9	58.1	245 bp	1.0	79.1
rpoA-rps11_p4	GGAGAGAGAATAAAGCGCCC	GAAGGGATGGAGCATTACGG	55.0	55.0	58.1	58.1	294 bp	1.0	78.9
rpoA-rps11_p5	GGAGAGAGAATAAAGCGCCC	GGAAGGGATGGAGCATTACG	55.0	55.0	58.1	58.1	295 bp	1.0	78.9