Hotspot

rps16

LSC · 5409-6536 · 1128 bp

Back to Malvaviscus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1128
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGAATCATCCACACCCTCTT	TGGATCGACTCAACCTATGAC	45.0	47.6	56.1	56.9	1229 bp	1.0	48.4
rps16_p2	TGAATCATCCACACCCTCTTT	TGGATCGACTCAACCTATGAC	42.9	47.6	56.9	56.9	1229 bp	1.0	46.6
rps16_p3	ATGAATCATCCACACCCTCT	TGGATCGACTCAACCTATGAC	45.0	47.6	55.9	56.9	1230 bp	1.0	46.4
rps16_p4	TGAATCATCCACACCCTCTT	GGATCGACTCAACCTATGACT	45.0	47.6	56.1	56.6	1228 bp	1.0	46.1
rps16_p5	TGAATCATCCACACCCTCTT	TGGATCGACTCAACCTATGACT	45.0	45.5	56.1	58.4	1229 bp	1.0	46.0