Hotspot

matK

LSC · 2248-3762 · 1515 bp

Back to Malvaviscus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1515
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	CTCTTCATCATTTCGGATCGGA	AACCTACTTGTCTCCGAGGT	45.5	50.0	58.0	57.7	1697 bp	1.0	61.4
matK_p2	TCTCTTCATCATTTCGGATCGG	AACCTACTTGTCTCCGAGGT	45.5	50.0	58.0	57.7	1698 bp	1.0	61.4
matK_p3	TCTTCATCATTTCGGATCGGA	AACCTACTTGTCTCCGAGGT	42.9	50.0	56.9	57.7	1696 bp	1.0	60.8
matK_p4	TCTTCATCATTTCGGATCGGAA	AACCTACTTGTCTCCGAGGT	40.9	50.0	57.6	57.7	1696 bp	1.0	58.3
matK_p5	CTCTTCATCATTTCGGATCGG	AACCTACTTGTCTCCGAGGT	47.6	50.0	56.6	57.7	1697 bp	1.0	58.2