Hotspot

atpA

LSC · 10709-12232 · 1524 bp

Back to Malvaviscus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	GCCCGCTTACGAAAAGTAGA	50.0	50.0	58.0	58.0	2415 bp	1.0	79.8
atpA_p2	CCTAGCCTTCCAAGCTAACG	GCCCGCTTACGAAAAGTAGA	55.0	50.0	58.1	58.0	2418 bp	1.0	79.5
atpA_p3	AGCCTTCCAAGCTAACGATG	AATGGAGGCGGATCAGTTTC	50.0	50.0	58.0	58.0	2395 bp	1.0	79.4
atpA_p4	AGCCTTCCAAGCTAACGATG	GAAATGGAGGCGGATCAGTT	50.0	50.0	58.0	58.0	2397 bp	1.0	79.4
atpA_p5	CCTAGCCTTCCAAGCTAACG	AATGGAGGCGGATCAGTTTC	55.0	50.0	58.1	58.0	2398 bp	1.0	79.2