Hotspot

atpH-atpI

LSC · 14502-15664 · 1163 bp

Back to Malvaviscus

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1163
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	50.0	50.0	58.0	58.0	1255-1263 bp	1.0	79.5
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	1221-1229 bp	1.0	79.5
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	GCGAATCCATGGAAGGTCAT	50.0	50.0	57.9	58.0	1277-1285 bp	1.0	78.9
atpH-atpI_p4	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1284-1292 bp	1.0	78.7
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1250-1258 bp	1.0	78.6