Hotspot

rpl32-trnL-UAG

SSC · 116976-118147 · 1172 bp

Back to Malva

Pi 0.0116
MarkerSeek score 57.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1172
variable_sites
28
pi
0.0116
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGGCGTTGAAAGCTTTTTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1115-1287 bp 1.0 84.7
rpl32-trnL-UAG_p2 CGGCGTTGAAAGCTTTTTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1116-1288 bp 1.0 84.7
rpl32-trnL-UAG_p3 CGGCGTTGAAAGCTTTTTCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 1125-1297 bp 1.0 83.7
rpl32-trnL-UAG_p4 AAGAAAGGATATTGGGCGGC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 1131-1303 bp 1.0 83.5
rpl32-trnL-UAG_p5 AAGAAAGGATATTGGGCGGC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 1132-1304 bp 1.0 83.5