Hotspot

trnN-GUU-ndhF

SSC · 112216-113436 · 1221 bp

Back to Malva

Pi 0.0118
MarkerSeek score 62.10
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
SSC
length_bp
1221
variable_sites
28
pi
0.0118
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 AGGATTCGAACCTACGACCA GAGGAAGTCGCATCTCGTTT 50.0 50.0 58.2 58.0 1366-1386 bp 1.0 82.1
trnN-GUU-ndhF_p2 AGGATTCGAACCTACGACCA TGTAGGAGGAAGTCGCATCT 50.0 50.0 58.2 57.9 1371-1391 bp 1.0 81.0
trnN-GUU-ndhF_p3 CTCTACCGCTGAGCTACTGA GAGGAAGTCGCATCTCGTTT 55.0 50.0 58.3 58.0 1327-1347 bp 1.0 80.8
trnN-GUU-ndhF_p4 TCTACCGCTGAGCTACTGAG GAGGAAGTCGCATCTCGTTT 55.0 50.0 58.3 58.0 1326-1346 bp 1.0 80.8
trnN-GUU-ndhF_p5 TCTACCGCTGAGCTACTGAG TGTAGGAGGAAGTCGCATCT 55.0 50.0 58.3 57.9 1331-1351 bp 1.0 79.7