Hotspot

psaJ-rpl33

LSC · 69644-70164 · 521 bp

Back to Malva

Pi 0.00978
MarkerSeek score 58.50
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
521
variable_sites
11
pi
0.00978
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTCCCGGATGCGTTAACATT ATACCTGGAAATGCCCGTTG 45.0 50.0 57.9 58.2 642-653 bp 1.0 80.0
psaJ-rpl33_p2 TTCCCGGATGCGTTAACATT CTGGAAATGCCCGTTGATTG 45.0 50.0 57.9 57.7 638-649 bp 1.0 79.7
psaJ-rpl33_p3 TTCCCGGATGCGTTAACATT GAAATGCCCGTTGATTGCTT 45.0 45.0 57.9 57.6 635-646 bp 1.0 79.0
psaJ-rpl33_p4 GTTTCTTCCCGGATGCGTTA ATACCTGGAAATGCCCGTTG 50.0 50.0 58.3 58.2 647-658 bp 1.0 78.8
psaJ-rpl33_p5 TTCTTCCCGGATGCGTTAAC ATACCTGGAAATGCCCGTTG 50.0 50.0 58.3 58.2 645-656 bp 1.0 78.8