Hotspot

petA-psbJ

LSC · 65821-66970 · 1150 bp

Back to Malva

Pi 0.0143
MarkerSeek score 57.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1150
variable_sites
31
pi
0.0143
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CTACGTGTCCAAGGCCTTTT GCTGGTATTCCTGTGATCGG 50.0 55.0 58.1 58.1 1324-1339 bp 1.0 81.1
petA-psbJ_p2 CTACGTGTCCAAGGCCTTTT ACGGTAGCTGGTATTCCTGT 50.0 50.0 58.1 58.1 1330-1345 bp 1.0 81.0
petA-psbJ_p3 GGCCTTTTGTTCTTCTTGGC GCTGGTATTCCTGTGATCGG 50.0 55.0 57.8 58.1 1312-1327 bp 1.0 80.8
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC ACGGTAGCTGGTATTCCTGT 50.0 50.0 57.8 58.1 1318-1333 bp 1.0 80.7
petA-psbJ_p5 CTACGTGTCCAAGGCCTTTT CGGTAGCTGGTATTCCTGTG 50.0 55.0 58.1 57.8 1329-1344 bp 1.0 80.3