Hotspot

trnF-GAA-ndhJ

LSC · 50433-51186 · 754 bp

Back to Malva

Pi 0.00743
MarkerSeek score 58.90
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
754
variable_sites
11
pi
0.00743
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA CCCGAAAGTTGGATAGGGTG 50.0 55.0 58.1 58.0 843-965 bp 1.0 82.7
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA CCGAAAGTTGGATAGGGTGG 50.0 55.0 58.1 58.0 842-964 bp 1.0 82.7
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA GAAAGTTGGATAGGGTGGCC 50.0 55.0 58.1 58.2 840-962 bp 1.0 81.2
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA ATGCCCGAAAGTTGGATAGG 50.0 50.0 58.1 57.7 846-968 bp 1.0 80.2
trnF-GAA-ndhJ_p5 TCGGGATAGCTCAGTTGGTA CCGAAAGTTGGATAGGGTGG 50.0 55.0 57.6 58.0 880-1002 bp 1.0 79.4