Hotspot

psbZ-trnG-GCC

LSC · 37589-38279 · 691 bp

Back to Malva

Pi 0.0105
MarkerSeek score 65.20
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
691
variable_sites
12
pi
0.0105
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.95
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 786-848 bp 1.0 82.2
psbZ-trnG-GCC_p2 GCTTCTCCTGATGGTTGGTT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 784-846 bp 1.0 82.2
psbZ-trnG-GCC_p3 CTCCTGATGGTTGGTTGAGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.1 57.8 780-842 bp 1.0 76.9
psbZ-trnG-GCC_p4 TGCTTCTCCTGATGGTTGGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.9 57.8 785-847 bp 1.0 76.4
psbZ-trnG-GCC_p5 TCTCCTGATGGTTGGTTGAG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 56.8 57.8 781-843 bp 1.0 74.5