Hotspot

trnT-GGU-psbD

LSC · 32784-34273 · 1490 bp

Back to Malva

Pi 0.00834
MarkerSeek score 66.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1490
variable_sites
23
pi
0.00834
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGGTCCCTACGTAACC 55.0 55.0 58.1 58.0 1566-1632 bp 1.0 84.6
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1569-1635 bp 1.0 84.4
trnT-GGU-psbD_p3 ACGGCCCTTTTAACTCAGTG GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1583-1649 bp 1.0 84.2
trnT-GGU-psbD_p4 AATTGAATCGAACGGCCCTT GAAACGGTCCCTACGTAACC 45.0 55.0 58.2 58.0 1594-1660 bp 1.0 83.8
trnT-GGU-psbD_p5 ATTGAATCGAACGGCCCTTT GAAACGGTCCCTACGTAACC 45.0 55.0 58.2 58.0 1593-1659 bp 1.0 83.8