Hotspot

rps16-trnQ-UUG

LSC · 6532-7127 · 596 bp

Back to Malva

Pi 0.00615
MarkerSeek score 63.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
596
variable_sites
8
pi
0.00615
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AGTGGTGGGTTTTGACATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 702-716 bp 1.0 81.4
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 663-677 bp 1.0 80.4
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 674-688 bp 1.0 79.9
rps16-trnQ-UUG_p4 GTGGTGGGTTTTGACATCCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.3 58.1 701-715 bp 1.0 79.0
rps16-trnQ-UUG_p5 TGGTGGGTTTTGACATCCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.3 58.1 700-714 bp 1.0 79.0