Hotspot

rpl32-trnL

SSC · 118004-119394 · 1391 bp

Back to Malus

Pi 0.00473
MarkerSeek score 58.30
Primer available yes
Label rpl32-trnL

Feature Metadata

label_name
rpl32-trnL
feature_type
igs
region
SSC
length_bp
1391
variable_sites
56
pi
0.00473
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1465-1603 bp 1.0 84.3
rpl32-trnL_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1466-1604 bp 1.0 84.3
rpl32-trnL_p3 TAGGGCAGCATTGAAAGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1467-1605 bp 1.0 83.9
rpl32-trnL_p4 TAGGGCAGCATTGAAAGCTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1468-1606 bp 1.0 83.9
rpl32-trnL_p5 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 1475-1613 bp 1.0 83.3