Hotspot

ndhF-rpl32

SSC · 116801-117844 · 1044 bp

Back to Malus

Pi 0.00755
MarkerSeek score 53.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1044
variable_sites
57
pi
0.00755
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAATGAAAGGT AAAAGCTTTCAATGCTGCCC 45.0 45.0 55.2 57.8 1189-1227 bp 1.0 62.7
ndhF-rpl32_p2 CTGGAAGTGGAATGAAAGGT AAGCTTTCAATGCTGCCCTA 45.0 45.0 55.2 57.8 1187-1225 bp 1.0 62.4
ndhF-rpl32_p3 CTGGAAGTGGAATGAAAGGT GCTGCCCTATATCCCTTCCT 45.0 55.0 55.2 58.3 1176-1214 bp 1.0 61.5
ndhF-rpl32_p4 CTGGAAGTGGAATGAAAGGT AGCTTTCAATGCTGCCCTAT 45.0 45.0 55.2 57.6 1186-1224 bp 1.0 60.5
ndhF-rpl32_p5 CTGGAAGTGGAATGAAAGGT TGCTGCCCTATATCCCTTCC 45.0 55.0 55.2 58.6 1177-1215 bp 1.0 59.1