Hotspot

rpl33-rps18

LSC · 71633-71850 · 218 bp

Back to Malus

Pi 0.00645
MarkerSeek score 52.60
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
218
variable_sites
12
pi
0.00645
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CGACACAATACGCCTAGTCG TCAATTCGATCCCCCGATTG 55.0 50.0 58.5 57.7 373-429 bp 1.0 80.7
rpl33-rps18_p2 GACACAATACGCCTAGTCGA TCAATTCGATCCCCCGATTG 50.0 50.0 57.2 57.7 372-428 bp 1.0 78.3
rpl33-rps18_p3 TCGACACAATACGCCTAGTC TCAATTCGATCCCCCGATTG 50.0 50.0 57.2 57.7 374-430 bp 1.0 78.3
rpl33-rps18_p4 CGACACAATACGCCTAGTCG ATTCGATCCCCCGATTGGAT 55.0 50.0 58.5 58.6 370-426 bp 1.0 77.7
rpl33-rps18_p5 AGAATCGACACAATACGCCT TCAATTCGATCCCCCGATTG 45.0 50.0 57.0 57.7 378-434 bp 1.0 77.3