Hotspot

petA-psbJ

LSC · 66631-67626 · 996 bp

Back to Malus

Pi 0.00502
MarkerSeek score 53.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
996
variable_sites
30
pi
0.00502
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGCTCTTCTTGGCATCTGTT AGCCGGTATTCTTGTGATCG 45.0 50.0 57.4 57.8 1154-1202 bp 1.0 77.4
petA-psbJ_p2 TTGCTCTTCTTGGCATCTGT AGCCGGTATTCTTGTGATCG 45.0 50.0 57.4 57.8 1155-1203 bp 1.0 77.4
petA-psbJ_p3 CGTGTCCAAGGTCTTTTGCT AGCCGGTATTCTTGTGATCG 50.0 50.0 58.7 57.8 1170-1218 bp 1.0 76.4
petA-psbJ_p4 GGTCTTTTGCTCTTCTTGGC AGCCGGTATTCTTGTGATCG 50.0 50.0 57.3 57.8 1161-1209 bp 1.0 76.2
petA-psbJ_p5 TCCATTACGTGTCCAAGGTC AGCCGGTATTCTTGTGATCG 50.0 50.0 57.2 57.8 1177-1225 bp 1.0 75.9