Hotspot

ndhC-trnV-UAC

LSC · 54280-54971 · 692 bp

Back to Malus

Pi 0.0161
MarkerSeek score 73.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
692
variable_sites
78
pi
0.0161
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.94
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 642-844 bp 1.0 89.3
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT GCACGTTGGGTCTTTGAAAC 50.0 50.0 57.9 58.2 706-908 bp 1.0 89.0
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AATGCACGTTGGGTCTTTGA 50.0 45.0 57.9 58.3 709-911 bp 1.0 87.8
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT ATGCACGTTGGGTCTTTGAA 50.0 45.0 57.9 58.3 708-910 bp 1.0 87.8
ndhC-trnV-UAC_p5 CTGGTCCTTTGCTAATCGGG GCACGTTGGGTCTTTGAAAC 55.0 50.0 58.3 58.2 723-925 bp 0.976744 85.8