Hotspot

psaA-ycf3

LSC · 44899-45601 · 703 bp

Pi 0.00183

MarkerSeek score 53.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 10
pi: 0.00183
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TGGAAACAAGCAATAGCGCT	50.0	45.0	57.9	58.5	848-883 bp	1.0	79.2
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	CAATAGCGCTTACTCCTGGT	50.0	50.0	57.9	57.4	838-873 bp	1.0	78.2
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	GGAGGCTTGGTTCGATCAAG	50.0	55.0	57.9	58.6	879-914 bp	1.0	77.8
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GCAATAGCGCTTACTCCTGG	50.0	55.0	57.9	58.8	839-874 bp	1.0	76.8
psaA-ycf3_p5	TTGAGAAATGACCCGGTCTG	TGGAAACAAGCAATAGCGCT	50.0	45.0	57.5	58.5	868-903 bp	1.0	76.3