Hotspot

psbZ-trnG-GCC

LSC · 39016-39521 · 506 bp

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Pi 0.0145
MarkerSeek score 64.40
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
506
variable_sites
80
pi
0.0145
unique_haplotype_count
31
primer_available
yes
alignment_reliability
0.92
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTTGCTTCTCCTGAGGGTTG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.7 57.8 564-742 bp 1.0 88.4
psbZ-trnG-GCC_p2 GGGTTGGTCGGGTAACAAAA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.3 57.8 550-728 bp 1.0 88.2
psbZ-trnG-GCC_p3 TTCCGGCACATCATTATGGA CGTCTTCTCCTTGGCAAAGA 45.0 50.0 57.0 57.8 520-698 bp 1.0 82.5
psbZ-trnG-GCC_p4 AGGGTTGGTCGGGTAACAAA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 59.2 57.8 551-729 bp 1.0 81.4
psbZ-trnG-GCC_p5 ATTTGCTTCTCCTGAGGGTTG CGTCTTCTCCTTGGCAAAGA 47.6 50.0 58.2 57.8 565-743 bp 1.0 81.0