Hotspot

atpH-atpI

LSC · 14521-15697 · 1177 bp

Back to Malus

Pi 0.00467
MarkerSeek score 63.70
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1177
variable_sites
36
pi
0.00467
unique_haplotype_count
33
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AATAGAAGCAAGCCCGACAG GCGAATCCATGGAAGGTCAT 50.0 50.0 58.0 58.0 1236-1270 bp 1.0 85.8
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT GCGAATCCATGGAAGGTCAT 45.0 50.0 58.0 58.0 1202-1236 bp 1.0 85.8
atpH-atpI_p3 AATAGAAGCAAGCCCGACAG TTTTTGCAACCTTAGCTGCG 50.0 45.0 58.0 57.9 1266-1300 bp 1.0 84.9
atpH-atpI_p4 ATAACGGAAGCGGCAGAAAT TTTTTGCAACCTTAGCTGCG 45.0 45.0 58.0 57.9 1232-1266 bp 1.0 84.9
atpH-atpI_p5 AATAACGGAAGCGGCAGAAA GCGAATCCATGGAAGGTCAT 45.0 50.0 58.2 58.0 1203-1237 bp 1.0 84.7