Hotspot

atpA-atpF

LSC · 12485-12538 · 54 bp

Back to Malus

Pi 0.00591
MarkerSeek score 53.20
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
54
variable_sites
4
pi
0.00591
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 ATGCCATCGCCTACTTGAAG TGGTATGTTTGGAACGGTGA 50.0 45.0 58.0 57.1 205-209 bp 1.0 74.9
atpA-atpF_p2 GCTCAATACGTTCGCGGATA TGGTATGTTTGGAACGGTGA 50.0 45.0 58.2 57.1 144-148 bp 1.0 74.5
atpA-atpF_p3 ATGCCATCGCCTACTTGAAG TGGTATGTTTGGAACGGTGAA 50.0 42.9 58.0 57.8 205-209 bp 1.0 72.7
atpA-atpF_p4 ATGCCATCGCCTACTTGAAG TTGGTATGTTTGGAACGGTGA 50.0 42.9 58.0 57.8 206-210 bp 1.0 72.7
atpA-atpF_p5 CAATGCCATCGCCTACTTGA TGGTATGTTTGGAACGGTGA 50.0 45.0 58.3 57.1 207-211 bp 1.0 72.6