Hotspot

ndhF

SSC · 118000-121579 · 3580 bp

Back to Mallotus

Pi 0.0318
MarkerSeek score 63.40
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
3580
variable_sites
135
pi
0.0318
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TGACCAGTTTGAGAATGCTGT TAGGAAGTGGTCGCATCTCT 42.9 50.0 57.8 57.9 387-442 bp 0.666667 67.8
ndhF_p2 TGACCAGTTTGAGAATGCTGT TTGGTCCTATAGCCGAGGTT 42.9 50.0 57.8 57.8 554-609 bp 0.666667 67.6
ndhF_p3 TGACCAGTTTGAGAATGCTGT GTAGGAAGTGGTCGCATCTC 42.9 55.0 57.8 57.8 388-443 bp 0.666667 67.3
ndhF_p4 TGACCAGTTTGAGAATGCTGT CCAATGGAGTCGGTGTTACC 42.9 55.0 57.8 58.3 438-493 bp 0.666667 66.8
ndhF_p5 TGACCAGTTTGAGAATGCTGT GGAAGTGGTCGCATCTCTTC 42.9 55.0 57.8 58.4 385-440 bp 0.666667 66.1