Hotspot

psbE-petL

LSC · 69204-70788 · 1585 bp

Back to Mallotus

Pi 0.101

MarkerSeek score 85.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1585
variable_sites: 186
pi: 0.101
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGCCACTAGTAGAAACCCGA	50.0	50.0	57.8	57.8	1430-1655 bp	1.0	88.9
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AGCCACTAGTAGAAACCCGA	55.0	50.0	58.2	57.8	1425-1650 bp	1.0	88.6
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	AGCCACTAGTAGAAACCCGA	50.0	50.0	58.4	57.8	1423-1648 bp	1.0	87.4
psbE-petL_p4	TTCTCCTGTGCTTCCAGACA	AGCCACTAGTAGAAACCCGA	50.0	50.0	58.6	57.8	1424-1649 bp	1.0	85.6
psbE-petL_p5	AAGAACGTTCTCCTGTGCTT	AGCCACTAGTAGAAACCCGA	45.0	50.0	57.4	57.8	1431-1656 bp	1.0	85.2