Hotspot

accD-psaI

LSC · 62557-63722 · 1166 bp

Back to Mallotus

Pi 0.0808

MarkerSeek score 82.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1166
variable_sites: 123
pi: 0.0808
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GTTACTTCGGCTCCACGATT	AGGGGAGCTGTCTGTATGTT	50.0	50.0	58.0	58.0	1487-1619 bp	1.0	90.9
accD-psaI_p2	GTTACTTCGGCTCCACGATT	GAGCCCAGCCTCCTCATATA	50.0	55.0	58.0	58.1	1659-1791 bp	1.0	90.5
accD-psaI_p3	GTTACTTCGGCTCCACGATT	TTTGAGGGGAGCTGTCTGTA	50.0	50.0	58.0	58.0	1491 bp	0.333333	53.1
accD-psaI_p4	CGCCTATATTGCATTTGCGG	TTTGAGGGGAGCTGTCTGTA	50.0	50.0	58.0	58.0	1641 bp	0.333333	52.9
accD-psaI_p5	TAACCGAAGCCGTTCAAACA	TTTGAGGGGAGCTGTCTGTA	45.0	50.0	58.0	58.0	1999 bp	0.333333	52.7