Hotspot

trnG-UCC-trnfM-CAU

LSC · 40567-40739 · 173 bp

Back to Mallotus

Pi 0.0694
MarkerSeek score 81.90
Primer available yes
Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name
trnG-UCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
173
variable_sites
18
pi
0.0694
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnfM-CAU_p1 TCTTTGCCAAGGAGAAGACG AACCTTGAAGTCACGGGTTC 50.0 50.0 57.8 58.0 254-278 bp 1.0 85.1
trnG-UCC-trnfM-CAU_p2 TCTTTGCCAAGGAGAAGACG CCTTGAAGTCACGGGTTCAA 50.0 50.0 57.8 58.0 252-276 bp 1.0 85.1
trnG-UCC-trnfM-CAU_p3 TCTTTGCCAAGGAGAAGACG AAGTCACGGGTTCAAATCCC 50.0 50.0 57.8 58.1 247-271 bp 1.0 84.8
trnG-UCC-trnfM-CAU_p4 TCTTTGCCAAGGAGAAGACG CTTCATTACGCGGGGTAGAG 50.0 55.0 57.8 57.9 299-323 bp 1.0 83.3
trnG-UCC-trnfM-CAU_p5 TCTTTGCCAAGGAGAAGACG CTCTTCATTACGCGGGGTAG 50.0 55.0 57.8 57.9 301-325 bp 1.0 83.2