Hotspot

psbZ-trnG-UCC

LSC · 39871-40495 · 625 bp

Back to Mallotus

Pi 0.109

MarkerSeek score 91.80

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 625
variable_sites: 92
pi: 0.109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 91.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	769-1072 bp	1.0	90.5
psbZ-trnG-UCC_p2	CTTCTCCTGATGGTTGGTCG	CTTTACCTTACCTTGGGCGG	55.0	55.0	58.0	58.3	808-1111 bp	1.0	89.8
psbZ-trnG-UCC_p3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	765-1068 bp	1.0	88.8
psbZ-trnG-UCC_p4	CTTCTCCTGATGGTTGGTCG	TTACCTTACCTTGGGCGGAT	55.0	50.0	58.0	58.4	806-1109 bp	1.0	88.6
psbZ-trnG-UCC_p5	TCCTGATGGTTGGTCGAGTA	CTTTACCTTACCTTGGGCGG	50.0	55.0	57.8	58.3	804-1107 bp	1.0	88.2