Hotspot

trnE-UUC-trnT-GGU

LSC · 32936-34705 · 1770 bp

Back to Mallotus

Pi 0.0783

MarkerSeek score 69.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1770
variable_sites: 97
pi: 0.0783
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.61
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	TCTACCACTGAGTTAAAAGGGC	55.0	45.5	58.3	57.7	1186-1840 bp	0.666667	54.9
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	TCTACCACTGAGTTAAAAGGGC	50.0	45.5	57.7	57.7	1165-1819 bp	0.666667	54.8
trnE-UUC-trnT-GGU_p3	TCGTAATACCCTACCCCCAG	TCTACCACTGAGTTAAAAGGGC	55.0	45.5	57.6	57.7	1222-1876 bp	0.666667	53.8
trnE-UUC-trnT-GGU_p4	CCTGAACCACTAGACGATGG	TCTACCACTGAGTTAAAAGGGC	55.0	45.5	57.4	57.7	1162-1816 bp	0.666667	52.4
trnE-UUC-trnT-GGU_p5	CTGAACCACTAGACGATGGG	TCTACCACTGAGTTAAAAGGGC	55.0	45.5	57.4	57.7	1161-1815 bp	0.666667	52.4