Hotspot

trnD-GUC-trnY-GUA

LSC · 32347-32728 · 382 bp

Back to Mallotus

Pi 0.0532
MarkerSeek score 83.50
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
382
variable_sites
30
pi
0.0532
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 452-555 bp 1.0 82.3
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 451-554 bp 1.0 82.3
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA AATTGCTTCTTCCTGGGTCG 50.0 50.0 58.5 58.2 510-613 bp 1.0 81.9
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA TGGAATTGCTTCTTCCTGGG 50.0 50.0 58.5 57.8 513-616 bp 1.0 81.5
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA GGAATTGCTTCTTCCTGGGT 50.0 50.0 58.5 57.8 512-615 bp 1.0 81.5