Hotspot

atpH-atpI

LSC · 15023-16021 · 999 bp

Back to Mallotus

Pi 0.0755

MarkerSeek score 98.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 105
pi: 0.0755
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 98.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCATGGAAGGGCATCATTGA	45.0	50.0	58.0	57.9	1051-1219 bp	1.0	90.6
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TCCATGGAAGGGCATCATTG	45.0	50.0	58.0	57.9	1052-1220 bp	1.0	90.6
atpH-atpI_p3	AGCGGCAGAAATCAATGGAT	CCATGGAAGGGCATCATTGA	45.0	50.0	57.9	57.9	1043-1211 bp	1.0	90.5
atpH-atpI_p4	AGCGGCAGAAATCAATGGAT	TCCATGGAAGGGCATCATTG	45.0	50.0	57.9	57.9	1044-1212 bp	1.0	90.5
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1086-1254 bp	1.0	89.9