Hotspot

rps16-trnQ-UUG

LSC · 6576-7658 · 1083 bp

Back to Mallotus

Pi 0.0803

MarkerSeek score 84.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1083
variable_sites: 118
pi: 0.0803
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TGGACTTGAAATTTGCCCGA	GAAATTCAAATGGGGCGTGG	45.0	50.0	58.0	58.0	1491-1646 bp	1.0	90.6
rps16-trnQ-UUG_p2	GCCCGAATTGGATCCTTTCT	GAAATTCAAATGGGGCGTGG	50.0	50.0	57.9	58.0	1477-1632 bp	1.0	90.3
rps16-trnQ-UUG_p3	TGGACTTGAAATTTGCCCGA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1433-1588 bp	1.0	90.3
rps16-trnQ-UUG_p4	TGGACTTGAAATTTGCCCGA	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.0	57.9	2033-2221 bp	1.0	88.8
rps16-trnQ-UUG_p5	TGGACTTGAAATTTGCCCGA	TAGGCTTCGGGCAATTTTGT	45.0	45.0	58.0	58.1	1946-2134 bp	1.0	88.8