Hotspot

rps16

LSC · 4942-6102 · 1161 bp

Back to Malcolmia

Pi 0.00172
MarkerSeek score 59.70
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1161
variable_sites
2
pi
0.00172
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 ACGAGTCTTTCACATCCTCT ACCCGTAAATTCACTATTTCATTCA 45.0 32.0 55.9 57.1 1314-1315 bp 1.0 40.1
rps16_p2 ACGAGTCTTTCACATCCTCT ACGATATACTGACTGAACTATGACT 45.0 36.0 55.9 57.0 1266-1267 bp 1.0 40.1
rps16_p3 ACGAGTCTTTCACATCCTCT ACCCGTAAATTCACTATTTCA 45.0 33.3 55.9 52.9 1314-1315 bp 1.0 40.1
rps16_p4 ACGAGTCTTTCACATCCTCTT ACCCGTAAATTCACTATTTCATTCA 42.9 32.0 56.7 57.1 1314-1315 bp 1.0 40.1
rps16_p5 ACGAGTCTTTCACATCCTCTT ACGATATACTGACTGAACTATGACT 42.9 36.0 56.7 57.0 1266-1267 bp 1.0 40.1